Identification of Candida species and investigating antifungal susceptibility in Turkey

WOS: 00020970400060

REV1 restrains DNA polymerase ζ to ensure frame fidelity during translesion synthesis of UV photoproducts in vivo

Exposure to ultraviolet light induces a number of forms of damage in DNA, of which (6–4) photoproducts present the most formidable challenge to DNA replication. No single DNA polymerase has been shown to bypass these lesions efficiently in vitro suggesting that the coordinate use of a number of different enzymes is required in vivo. To further understand the mechanisms and control of lesion bypass in vivo, we have devised a plasmid-based system to study the replication of site-specific T–T(6–4) photoproducts in chicken DT40 cells. We show that DNA polymerase ζ is absolutely required for translesion synthesis (TLS) of this lesion, while loss of DNA polymerase η has no detectable effect. We also show that either the polymerase-binding domain of REV1 or ubiquitinated PCNA is required for the recruitment of Polζ as the catalytic TLS polymerase. Finally, we demonstrate a previously unappreciated role for REV1 in ensuring bypass synthesis remains in frame with the template. Our data therefore suggest that REV1 not only helps to coordinate the delivery of DNA polymerase ζ to a stalled primer terminus but also restrains its activity to ensure that nucleotides are incorporated in register with the template strand

Radiolabeling efficiency and cell incorporation of chitosan nanoparticles

Cationic nanoparticles of CS were developed according to ionotropic gelation process as potential cancer cell targeting agent. CS nanoparticles (CSNP) (F1 and F2) diameters varied between ranges of 100 e800 nm. Particle size, polydispersity index and zeta potential values of formulations were measured by photon correlation spectroscopy. The morphological analysis for CSNPs was provided with scanning electron microscopy. For cell incorporation study, F1 and F2 were directly labeled by Technetium-99m (99mTc), radiochemical purity and stability of the complex were analyzed by radioactive thin layer chromatography and radioactive high performance liquid chromatography studies. After that, incorporation of 99mTc labeled F1 and F2 were evaluated in U2OS and NCIeH209 cell lines. The six well plates were used for all experiments and the integrity of each cell monolayer was checked by measuring its TEER values with an epithelial voltammeter. Results confirmed that F1 and F2 formulations were successfully radiolabeled with 99mTc. The incorporation percentages of 99mTc labeled F1 and F2 in NCIeH209 and U2OS cell lines were found different when they compared to 99mTc solution. Since 99mTc labeled F1 and F2 highly uptake in cancer cell line. The results demonstrated that radiolabeled CSNPs may be a promising agent for cancer diagnosis. © 2015 Elsevier B.V. All rights reserved

Radiolabeling and in vitro evaluation of 99mTc-methotrexate on breast cancer cell line

In the present study 99mTc-MTX was prepared with high labeling yield by a new simple and easy formulation method. According to cell culture studies, 99mTc-MTX incorporated with both MCF-7 and CRL8798 cells, with significant differences in the uptake percentages. Since 99mTc-MTX highly uptake in cancer cell line, the results demonstrated that radiolabeled MTX may be promising for breast cancer diagnosis of oncological patients. © 2015, Akadémiai Kiadó, Budapest, Hungary

Letter to the editor: Primary cardiac rhabdomyosarcoma

WOS: 000173435400022PubMed ID: 1181319

Nanovesicles for tumor-targeted drug delivery

Cancer is one of the most important burdens for the health systems worldwide due to cancer-related deaths associated with late diagnosis and treatment toxicities. Diagnosis of cancer plays a critical role in reducing cancer death rates as it facilitates early prognosis and treatment. Current cancer treatment mainly includes the resection of tumor tissue, radiation treatment, and pharmaceutical treatment. Although various techniques, methods, and drugs are clinically used in cancer diagnosis and treatment, they are inadequate for early diagnosis and effective treatment. Therefore, several studies regarding cancer-targeted nanosized drug delivery systems and theranostic approach are performed. Nanovesicular systems, one of the most important types of drug delivery systems, are formulated for the effective delivery and controlled release of active compounds such as drugs, genes, phytocompounds, and imaging agents. In this chapter, different types of nanovesicles are reviewed after providing information about conventional cancer imaging and treatment. Furthermore, targeting mechanisms of nanovesicles for cancer are explained. Finally, some studies performed to develop the nanovesicles as the imaging, treatment, or theranostic system for cancer are summarized. © 2022 Elsevier Inc. All rights reserved

Diagnosis, treatment and prognosis in cow's milk allergy

31st Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI) -- JUN 16-20, 2012 -- Geneva, SWITZERLANDWOS: 000310247703146European Acad Allergy & Clin Immunol (EAACI